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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in several tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two more splice variants have been reported but specific nucleotide sequence details isn't still out there.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide typically contains the epitope identified from the antibody.
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This guideline outlines additional feasible optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
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This guide outlines further more feasible optimization of cellular and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind on the epitope about the focus on protein. By evaluating the staining in the blocked antibody vs . the antibody alone, you can see which staining is distinct.
Concentrate on Facts Cyclin E belongs to your remarkably conserved cyclin spouse and children, whose associates are characterized by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation patterns which lead on the temporal coordination of each mitotic event. Cyclin E sorts a posh with and features as a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and it is degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring website the modulation of receptor activation by both equally antibodies...
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